RFP Antibody

SKU: orb334992

Description

Goat polyclonal antibody to RFP (Red fluorescent protein). RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Images & Validation

• Tested Applications: IEM, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB:1:500-1:2,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
• Application Notes:
  The antibody solution should be gently mixed before use.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Antigen: Purified recombinant RFP peptide produced in E. coli.. Antigen Sequence: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
• Target: Red Fluorescent Protein
• Purification: Epitope affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

Immunohistochemical analysis of formalin-fixed and paraffin-embedded epithelium cells using RFP antibody

Western blot analysis of staining of WB293HEK cells transduced with tdTomato Ad lysates using RFP antibody

Immunofluorescence analysis of 293HEK cells using RFP antibody

Quick Database Links

Gene Symbol

Red Fluorescent Protein

References

Krishnapriya Hari et al. GABA facilitates spike propagation through branch points of sensory axons in the spinal cord Nat Neurosci, 25(10), 1288-1299 (2022)

PubMed: 36163283

Applications

IHC

Reactivity

Rat

Shihao Lin et al. Locomotor-related propriospinal V3 neurons produce primary afferent depolarization and modulate sensory transmission to motoneurons J Neurophysiol, 130(4), 799-823 (2023)

PubMed: 37609680

Applications

IHC

Reactivity

Mouse

Zuobao Lin et al. Distinct mammary stem cells orchestrate long-term homeostasis of adult mammary gland Cell Discov, (2025)

PubMed: 40234382

Applications

IF