RFP Antibody

SKU: orb334992

Description

Goat polyclonal antibody to RFP (Red fluorescent protein). RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Images & Validation

• Tested Applications: IEM, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB:1:500-1:2,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
• Application Notes:
  The antibody solution should be gently mixed before use.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Antigen: Purified recombinant RFP peptide produced in E. coli. Antigen Sequence: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
• Target: Red Fluorescent Protein
• Purification: Epitope affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Concentration: Please inquire
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

Immunohistochemical analysis of formalin-fixed and paraffin-embedded epithelium cells using RFP antibody

Western blot analysis of staining of WB293HEK cells transduced with tdTomato Ad lysates using RFP antibody

Immunofluorescence analysis of 293HEK cells using RFP antibody

Quick Database Links

Gene Symbol

Red Fluorescent Protein

Krishnapriya Hari et al. GABA facilitates spike propagation through branch points of sensory axons in the spinal cord Nat Neurosci, 25(10), 1288-1299 (2022)

PubMed: 36163283

Applications

IHC

Reactivity

Rat

Shihao Lin et al. Locomotor-related propriospinal V3 neurons produce primary afferent depolarization and modulate sensory transmission to motoneurons J Neurophysiol, 130(4), 799-823 (2023)

PubMed: 37609680

Applications

IHC

Reactivity

Mouse

Zuobao Lin et al. Distinct mammary stem cells orchestrate long-term homeostasis of adult mammary gland Cell Discov, (2025)

PubMed: 40234382

Applications

IF

Shuaibo Huang et al. Cre Driver Efficiency in Myeloid Lineages Across Tissues and in a Model of Myocardial Injury FASEB J, (2026)

PubMed: 41575195

Applications

IF

Reactivity

Mouse

K. Metz, K. Hari et al. Reduced GABAB receptor activation and post activation depression of proprioceptive afferents after spinal cord injury bioRxiv,, (2026)

PubMed:

Applications

IHC

Reactivity

Mouse