tdTomato antibody

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Product Name tdTomato antibody
Catalog Number orb182397
Species/HostGoat
ConjugationUnconjugated
Tested applicationsICC, IF, IHC-Fr, IHC-P, WB
Immunogen

Recombinant peptide produced in E. coli.

Target tdTomato. In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (orb182397) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).
Alternative Names
Form/Appearance PBS, 10% glycerol and 0.03% sodium azide.
Concentration

- 100 µg size = 0.9 mg per ml

- 600 µg size = 3.0 mg per ml

Storage Store at 2-8 °C for one-two days. For extended storage, store in -20 °C freezer. Working dilution samples should be discarded if not used within 12 hours.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity

This antibody is epitope-affinity purified from goat antiserum.

MW 55 kDa
Dilution Range WB: 1:500-2000, IF/ICC: 1:50-500, IHC-P: 1:50-500, IHC-Fr:1:50-500
Application Notes Reacts against tdTomato, mCherry, red fluorescent protein (DsRed) from so-called disc corals of Discosoma species.
Description

Goat polyclonal antibody to tdTomato

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Western blot analysis of HEK293 cell line lysate using tdTomato antibody.
Western blot analysis of HEK293 cell line lysate using tdTomato antibody.
Confocal immunofluoroscence analysis of HEK293 cells using tdTomato antibody.
Confocal immunofluoroscence analysis of HEK293 cells using tdTomato antibody.