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    tdTomato antibody

    Catalog Number: orb182397

    Instock
    In stock
    $ 228.00
    Catalog Numberorb182397
    CategoryAntibodies
    DescriptionGoat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IF, IHC-Fr, IHC-P, WB
    ReactivityOther
    IsotypeIgG
    ImmunogenPurified recombinant peptide produced in E. coli.
    Concentration1 mg/ml
    Dilution rangeWB 1:500-1:2,000 IHC (F) 1:50-1:500 IHC (P) 1:50-1:500 IF 1:50-1:500
    PurityThis antibody is epitope-affinity purified from goat antiserum
    ConjugationUnconjugated
    MW55 kDa
    TargettdTomato
    Protein SequenceMEQKLISEEDLGTISTMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSNGGMDEVYK
    RRIDAB_2687917
    StorageFor continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours
    Buffer/PreservativesPBS, 20% glycerol and 0.05% sodium azide
    Alternative namesCherry fluorescent protein, DsRed, mCherry, red fl
    Read more...
    NoteFor research use only
    Application notesIn 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein)
    Expiration Date12 months from date of receipt.

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    IF

    Reactivity

    Mouse

    Li Li # 1 2 3, Yu He # 1 2 3, Kai Liu 4, Lin Liu 1 2 3, Shan Shan 1 2 3, Helin Liu 1 2 3, Jiangbo Ren 1 2 3, Shujie Sun 1 2 3, Min Wang 1 2 3, Jidong Jia 5 6 7, Ping Wang GITRL impairs hepatocyte repopulation by liver progenitor cells to aggravate inflammation and fibrosis by GITR+CD8+ T lymphocytes in CDE Mice Cell Death Dis, 15, 114 (2024)

    Applications

    IF

    Reactivity

    Mouse

    Jia Sun #1, Xinyuan Wang #12, Rui Sun 1, Xiaoao Xiao 1, Yu Wang 1, Yu Peng 1, Yuanqing Gao 3 Microglia shape AgRP neuron postnatal development via regulating perineuronal net plasticity Mol Psychiatry, (2023)

    Applications

    IF

    Reactivity

    Mouse

    Jian Ge 1, Hongxia Shao 1 2, Hongxu Ding 3, Yuefeng Huang 4, Xuebing Wu 5, Jie Sun 6, Jianwen Que Single Cell Analysis of Lung Lymphatic Endothelial Cells and Lymphatic Responses during Influenza Infection J Respir Biol Transl Med, 1, 10003 (2024)

    Applications

    IF

    Reactivity

    Mouse

    Applications

    IF

    Fang, Yinshan et al. Epithelial Wntless regulates postnatal alveologenesis Development, 149, dev199505 (2022)

    Applications

    IF, IHC

    Reactivity

    Mouse
    tdTomato antibody

    Western blot analysis of HEK293 cell line lysate using tdTomato antibody.

    tdTomato antibody

    Confocal immunofluoroscence analysis of HEK293 cells using tdTomato antibody.

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      IEM,  IF,  IHC-Fr,  IHC-P,  WB

      Other

      Goat

      Polyclonal

      Unconjugated

      100 μg
    • RFP antibody [orb334992]

      IEM,  IF,  IHC-Fr,  IHC-P,  WB

      Goat

      Polyclonal

      Unconjugated

      100 μg
    • RFP antibody [orb345939]

      Other

      1 Each
    • mCherry antibody [orb153320]

      IEM,  IF,  IHC-Fr,  IHC-P,  WB

      Other

      Goat

      Polyclonal

      Unconjugated

      100 μg
    • Carrier-free (BSA/glycerol-free) tdTomato antibody [orb1338793]

      WB

      Mouse

      Monoclonal

      Unconjugated

      100 μg
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