RFP antibody

• Catalog Number: orb344410
• Category: Antibodies
• Description: RFP antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8E5.G7
• Tested applications: ELISA, FC, IF, IP, WB
• Reactivity: Other
• Isotype: IgG2a
• Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
• Concentration: 1.02 mg/ml
• Dilution range: ELISA: 1:75,000 - 1:150,000, FC: User Optimized, IF: User Optimized, IP: User Optimized, WB: 1:1,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
• Conjugation: Unconjugated
• UniProt ID: Q9U6Y8
• Storage: Store anti-RFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-RFP Antibody, DsRed, rDsRed, Discosoma
• Note: For research use only
• Application notes: Anti-RFP antibody has been tested by ELISA, SDS-Page, and Western blot and is designed to detect Red Fluorescent Protein and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

References

Clarkson, Rebecca L. et al. D3 Receptors Regulate Excitability in a Unique Class of Prefrontal Pyramidal Cells J Neurosci, 37, 5846-5860 (2017)

PubMed: 28522735

Girasole, Allison E. et al. A Subpopulation of Striatal Neurons Mediates Levodopa-Induced Dyskinesia Neuron, 97, 787-795.e6 (2018)

PubMed: 29398356