Anti-PGC1 alpha Antibody

• Catalog Number: orb667759
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PGC1 alpha
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide of human PGC1 alpha. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PPARGC1A
• Entrez: 19017, 10891, 83516
• UniProt ID: Q9QYK2, Q9UBK2, O70343
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-LEM6 antibody, anti-PGC1 antibody, anti-PGC1A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ehsan Arabzadeh 1, Amir Sarshin 2, Foad Feizolahi 2, Majid Mohabbat 3, Mohammad-Ali Soleiman-Fallah 4, Alireza Rahimi 5, Anatoli Petridou 6, Zahra Emami 5, Helena Tajik 5, Reza Bozorg Omid 5, Amir Maleki 5, Hamid Ekrami Ogholbag 7, Ali Khademi 8, Mehdi Zargani Synergistic salvation: HIIT and herbal allies reverse NAFLD damage in rats J Mol Histol ., (2025)

PubMed: 40186827

Applications

IHC

Reactivity

Rat

Western blot analysis of PGC1 alpha expression in mouse heart (A), rat heart (B) whole cell lysates. (Predicted band size: 14; 30; 31; 33; 77; 89; 91 kD; Observed band size: 91 kD)

Immunohistochemical analysis of PGC1 alpha staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of PGC1 alpha staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.