PGC1 alpha + beta Rabbit Polyclonal Antibody

SKU: orb100963

Description

PGC1 alpha + beta Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PGC1 alpha + beta (151-250/798aa)
• Target: PPARGC1A
• Molecular Weight: 88/113 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

LEM6; Ligand effect modulator 6; PERC; Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; Peroxisome proliferative activated receptor, gamma, coactivator 1; Peroxisome proliferator activated receptor gamma coactivator 1 alpha; peroxisome proliferator-activated receptor gamma coactivator 1 beta; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Peroxisome proliferator-activated receptor gamma coactivator 1-beta; peroxisome proliferator-activated receptor gamma, coactivator 1 beta; PGC 1(alpha); PGC 1 alpha; PGC 1v; PGC-1(beta); PGC-1-alpha; PGC-1-beta; PGC-1-related estrogen receptor alpha coactivator; PGC1; PGC1(alpha); PGC1A; PGC1v; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPAR gamma coactivator-1beta; PPAR-gamma coactivator 1-alpha; PPAR-gamma coactivator 1-beta; PPARGC 1 alpha; PPARGC-1-alpha; PPARGC-1-beta; PPARGC1; PPARGC1A; Ppargc1b; PRGC1_HUMAN; PRGC2_HUMAN.

Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-PGC1 alpha+beta antibody (orb100963), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PGC1 alpha + beta) Polyclonal Antibody, Unconjugated (orb100963) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A673 (Human) Cell Lysate at 30 ug, Primary: Anti-PGC1 alpha + beta (orb100963) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 88/113 kD, Observed band size: 113 kD.

Sample: Lane 1: Molt-4 (Human) Cell Lysate at 30 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: Hela (Human) Cell Lysate at 30 ug, Lane 5: Jurkat (Human) Cell Lysate at 30 ug, Lane 6: HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-PGC1 alpha + beta (orb100963) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90/113 kD, Observed band size: 113 kD.

Sample: Muscle (Pig) Lysate at 40 ug, Kidney (Mouse) Lysate at 40 ug, Primary: Anti-PGC1 alpha + beta at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 88/113 kD, Observed band size: 92 kD.

Quick Database Links

Gene Symbol

PPARGC1A

UniProt

Q9UBK2