PPARGC1A Rabbit Polyclonal Antibody

SKU: orb1974626

Description

PPARGC1A Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: IHC-P=1:100-500, WB=1:500-2000, ICC/IF=1:50-100, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Goat, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human PPARGC1A (11-280/798aa)
• Target: PPARGC1A
• Molecular Weight: 88 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

LEM6; PGC-1 alpha; PGC1 alpha; Ligand effect modulator 6; Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; Peroxisome proliferative activated receptor, gamma, coactivator 1; Peroxisome proliferator activated receptor gamma coactivator 1 alpha; PGC 1(alpha); PGC 1 alpha; PGC 1v; PGC1; PGC1(alpha); PGC1A; PGC1v; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPARGC 1 alpha.

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PPARGC1A) polyclonal Antibody, Unconjugated (orb1974626) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human kidney carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (PPARGC1A) Polyclonal Antibody, Unconjugated (orb1974626) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (PPARGC1A) Polyclonal Antibody, Unconjugated (orb1974626) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Recombinant human PPARGC1A, N-Trx-His, Primary: Anti-PPARGC1A (orb1974626) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 88 kDa, Observed band size: 61 kDa.

Quick Database Links

Gene Symbol

PPARGC1A

UniProt

Q9UBK2