PPARGC1A Rabbit Polyclonal Antibody

SKU: orb1974626

Description

PPARGC1A Rabbit Polyclonal Antibody

Research Area

Cardiovascular Research, Cell Biology, Coactivators and Corepressors, DNA and RNA Research, Diabetes Research, Energy Metabolism, Epigenetics and Nuclear Signaling, Fatty Acid Oxidation, Fatty Acids, Metabolism, Mitochondrial, Mitochondrial Markers, Obesity

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: IHC-P=1:100-500, WB=1:500-2000, ICC/IF=1:50-100, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Goat, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human PPARGC1A (11-280/798aa)
• Target: PPARGC1A
• Molecular Weight: 88 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

LEM6; PGC-1 alpha; PGC1 alpha; Ligand effect modulator 6; Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; Peroxisome proliferative activated receptor, gamma, coactivator 1; Peroxisome proliferator activated receptor gamma coactivator 1 alpha; PGC 1(alpha); PGC 1 alpha; PGC 1v; PGC1; PGC1(alpha); PGC1A; PGC1v; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPARGC 1 alpha.

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PPARGC1A) polyclonal Antibody, Unconjugated (orb1974626) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human kidney carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (PPARGC1A) Polyclonal Antibody, Unconjugated (orb1974626) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (PPARGC1A) Polyclonal Antibody, Unconjugated (orb1974626) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Recombinant human PPARGC1A, N-Trx-His, Primary: Anti-PPARGC1A (orb1974626) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 88 kDa, Observed band size: 61 kDa.

Quick Database Links

Gene Symbol

PPARGC1A

UniProt

Q9UBK2