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Catalog Number | orb1562577 |
---|---|
Category | Antibodies |
Description | IL-17A Antibody |
Clonality | Monoclonal |
Clone Number | 9F9 |
Tested applications | ELISA, FC, ICC, IP |
Reactivity | Human |
Isotype | Mouse IgG1 kappa |
Immunogen | mammalian-derived human IL-17-IgG fusion protein, boost with recombinant human IL-17A |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Target | IL-17A |
Entrez | 3605 |
UniProt ID | Q16552 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Interleukin 17 Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 0.5-4 µg/ml. Intracellular staining. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor intracellular staining of PHA stimulated and Brefeldin A treated peripheral whole blood showing lymphocytes stained using anti-human CD4 (MEM-241) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human IL-17A (9F9) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).
Separation of human CD4 positive IL-17A positive lymphocytes (red-filled) from CD4 negative IL-17A negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated and Brefeldin A treated peripheral whole blood stained using anti-human IL-17A (9F9) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).
Flow cytometry intracellular staining pattern of human PHA stimulated and Brefeldin A treated peripheral whole blood stained using anti-human IL-17A (9F9) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).