Cart summary

You have no items in your shopping cart.

Anti-IL17/Il17a Antibody

Catalog Number: orb402354

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb402354
CategoryAntibodies
DescriptionAnti-IL17/Il17a Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenE. coli-derived mouse IL17 recombinant protein (Position: A26-A158).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW27 kDa
UniProt IDQ62386
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesInterleukin-17A; IL-17; IL-17A; Cytotoxic T-lympho
Read more...
NoteFor research use only
Application notesWestern blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml ELISA (Cap), 1-5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-IL17/Il17a Antibody

IHC analysis of IL17 using anti-IL17 antibody. IL17 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-IL17 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-IL17/Il17a Antibody

IHC analysis of IL17 using anti-IL17 antibody. IL17 was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-IL17 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-IL17/Il17a Antibody

IHC analysis of IL17 using anti-IL17 antibody. IL17 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-IL17 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-IL17/Il17a Antibody

Sandwich ELISA - Recombinant mouse IL17/Il17a protein standard curve. Use in combination with reagents from Mouse IL17/Il17a ELISA Kit EZ-Set (DIY Antibody Pairs).

Anti-IL17/Il17a Antibody

Western blot analysis of IL17 using anti-IL17 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human Jurkat cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL17 at approximately 27KD. The expected band size for IL17 is at 17KD.

  • Anti-IL17/IL17A Antibody [orb76367]

    ELISA,  WB

    Mouse

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-IL17/IL17A Antibody [orb2580325]

    ELISA,  WB

    Mouse

    Rabbit

    Polyclonal

    iFluor647

    100 μg
  • Anti-IL17/IL17A Antibody [orb2580326]

    ELISA,  WB

    Mouse

    Rabbit

    Polyclonal

    PE

    100 μg
  • Anti-IL17/IL17A Antibody [orb2580327]

    ELISA,  WB

    Mouse

    Rabbit

    Polyclonal

    APC

    100 μg
  • Anti-IL17/IL17A Antibody [orb2580328]

    ELISA,  WB

    Mouse

    Rabbit

    Polyclonal

    HRP

    100 μg