IL-17A Antibody (FITC)

• Catalog Number: orb1729628
• Category: Antibodies
• Description: Interleukin 17A (IL-17A) is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. It plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. It is the signature effector cytokine of Th17 cells, and in this role it primarily induces neutrophil activation and recruitment at infection and inflammatory sites. High levels of IL-17A are associated with rheumatoid arthritis, psoriasis, multiple sclerosis, and another inflammatory diseases, including lung injugy during severe COVID 19. This cytokine also contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. It is an effector cytokine for invariant NKT cells (iNKT), and it is involved in epithelial barrier formation upon injury.
• Target: IL-17A
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: FITC
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: mammalian-derived human IL-17-IgG fusion protein, boost with recombinant human IL-17A
• UniProt ID: Q16552
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
• Clone Number: 9F9
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: Interleukin 17
• Note: For research use only
• Entrez: 3605
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of PHA stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood) and intracellular staining using anti-human IL-17A (9F9) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD17A positive CD3 positive lymphocytes (red-filled) from CD17A negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-17A (9F9) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry intracellular staining pattern of PHA stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-17A (9F9) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).