CD314 Antibody

• Catalog Number: orb154374
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD314 which is also known as BAT221, NKG2D a cell surface receptor of the type II transmembrane receptor NKG2 family. CD314 is expressed on NK cells and some T cell subsets, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion.
• Target: CD314
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: NKL cell line
• UniProt ID: P26718
• Tested applications: FA, FC, IHC-Fr, IP
• Application notes: Functional application: Blocking of ligand binding. Immunohistochemistry: Recommended dilution: 5-10 μg/ml. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Antibody Type: Primary Antibody
• Clone Number: 1D11
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NKG2D, KLRK1, KLR
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Entrez: 22914
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD314 (1D11) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC and anti-human CD56 (LT56) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD314 (1D11) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC.