NKG2D Rabbit Polyclonal Antibody

• Catalog Number: orb11136
• Category: Antibodies
• Description: NKG2D Rabbit Polyclonal Antibody
• Target: KLRK1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from rat NKG2D (121-219/219aa)
• UniProt ID: O70215
• RRID: AB_10746403
• MW: 31-35 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=2μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NKG2-D type II integral membrane protein; KLRK1; n
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Mouse thymus cells (blue). Primary Antibody (green line): Rabbit Anti-NKG2D antibody (orb11136), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% methanol (Overnight at 4°C). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: SW480. Primary Antibody (green line): Rabbit Anti-NKG2D antibody (orb11136), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-NKG2D (orb11136) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kD, Observed band size: 26 kD.