NKG2D Rabbit Polyclonal Antibody

• Catalog Number: orb499571
• Category: Antibodies
• Description: NKG2D Rabbit Polyclonal Antibody
• Target: KLRK1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from mouse NKG2D (1-100/232aa)
• UniProt ID: O54709
• MW: 25 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KLRK1; natural killer cell group 2D; NKG2-D isofor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Mouse thymus cells (fixed with 70% methanol (Overnight at 4°C)). Primary Antibody (green line): Rabbit Anti-NKG2D antibody (orb499571), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-NKG2D (orb499571) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kD, Observed band size: 25 kD.

Sample: Lung Cell (Mouse) Lysate at 40 ug, Primary: Anti-NKG2D (orb499571) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kD, Observed band size: 25 kD.

Sample: Stomach (Mouse) Lysate at 40 ug, Primary: Anti-NKG2D (orb499571) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kD, Observed band size: 25 kD.