NKG2D Rabbit Polyclonal Antibody

• Catalog Number: orb500732
• Category: Antibodies
• Description: NKG2D Rabbit Polyclonal Antibody
• Target: KLRK1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Human, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human NKG2D (101-200/219aa)
• UniProt ID: P26718
• MW: 25 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KLRK1; natural killer cell group 2D; NKG2-D isofor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Mouse thymus cells (fixed with 70% methanol (Overnight at 4°C). Cells stained with Primary Antibody for 30 min at room temperature. The cells). Primary Antibody (green line): Rabbit Anti-NKG2D antibody (orb500732), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Sample: Lung (Mouse) Lysate at 40 ug, Raw246.7 (Mouse) Cell Lysate at 40 ug, Spleen (Mouse) Lysate at 40 ug, Lymph node (Mouse) Lysate at 40 ug, Bone (Rat) Lysate at 40 ug, Primary: Anti-NKG2D (orb500732) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kD, Observed band size: 25 kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-NKG2D (orb500732) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25kD, Observed band size: 26kD.