CD274 Antibody

SKU: orb545693

Description

Mouse monoclonal antibody to CD274

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FA, FC, IHC-Fr
• Reactivity: Human, Primate
• Application Notes:
  Functional application: Blocking. Immunohistochemistry (frozen sections): Acetone fixation. Flow cytometry: Recommended dilution: 1-4 µg/ml

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Clone No.: 29E.2A3
• Immunogen: Full length human CD274
• Target: CD274
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B7H1, PDL1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, B7-H1

Separation of cells stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 μg/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated peripheral blood mononuclear cell suspension.

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD274

UniProt

Q9NZQ7

NCBI Gene ID

29126