Anti-HMGCR Antibody

• Catalog Number: orb340767
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HMGCR
• Target: HMGCR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant full length protein of human HMGCR
• UniProt ID: Q01237, P51639, P04035
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1:2000, IF/ICC: 1:50-1:200, IHC-P: 1:50-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 3-hydroxy-3-methylglutaryl-coenzyme A reductase; H
• Note: For research use only
• Entrez: 25675, 15357, 3156

References

Kumari, Deepa et al. Hsp40s play distinct roles during the initial stages of apolipoprotein B biogenesis Mol Biol Cell, (2021)

PubMed: 34910568

Western blot analysis of HMGCR expression in HepG2 (A), Hela (B), mouse spleen (C), rat liver (D) whole cell lysates. (Predicted band size: 92; 97; 99 kD; Observed band size: 97 kD)

Immunohistochemical analysis of HMGCR staining in human small intestine formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.