HMGCR Antibody

SKU: orb340767

Description

The HMGCR Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human HMGCR .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB: 1:500-1:2000, IF/ICC: 1:50-1:200, IHC-P: 1:50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human HMGCR
• Purification: The antibody was purified by immunogen affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMG-CoA reductase

Western blot analysis of HMGCR expression in HepG2 (A), Hela (B), mouse spleen (C), rat liver (D) whole cell lysates. (Predicted band size: 92; 97; 99 kD; Observed band size: 97 kD)

Immunohistochemical analysis of HMGCR staining in human small intestine formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P51639, Q01237, P04035

NCBI Gene ID

15357, 25675, 3156

References

Kumari, Deepa et al. Hsp40s play distinct roles during the initial stages of apolipoprotein B biogenesis Mol Biol Cell, (2021)

PubMed: 34910568