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Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody

Catalog Number: orb99303

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb99303
CategoryAntibodies
DescriptionPhospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody
TargetPRKAA2
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 2 around the phosphorylation site of Thr172 LR(p-T)SC
UniProt IDP54646
MW64 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAMPK alpha 1(phospho T183)+AMPK alpha 2(phospho T1
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NoteFor research use only

Melo B.P. 1 , Zacarias A.C. 1 , Oliveira J.C.C. 1 , Cordeiro L.M.S. 2 , Horta N.A.C. 3 , Poletini M.O. 3 , Tonoli C. 4 , Dos Santos M.L. 5 , Wanner S.P. 1 , Meeusen R. 8 , Heyman E. 6 7 , Avelar G.F. 5 , Soares D.D Enhancing Metabolic and Inflammatory Status in Insulin-Resistant Rats: Acute Intervention with Cocoa Flavanols and Submaximal Aerobic Exercise Activates Intracellular Signaling Pathways for Glucose Metabolism Clinical Nutrition, (2024)

Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

  • Phospho-AMPK alpha 1 (Thr183) + AMPK alpha 2 (Thr172) Rabbit Polyclonal Antibody [orb182418]

    FC,  IF,  IHC-Fr,  IHC-P

    Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep

    Human, Mouse, Rat

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    100 μl, 200 μl, 50 μl
  • Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody (FITC) [orb8540]

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    100 μl
  • Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody (BF647) [orb1587525]

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    100 μl
  • Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody (HRP) [orb503233]

    IHC-Fr,  IHC-P

    Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    HRP

    100 μl