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AMPK alpha 1 Rabbit Polyclonal Antibody

Catalog Number: orb10076

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10076
CategoryAntibodies
DescriptionAMPK alpha 1 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P
Predicted ReactivityBovine, Canine, Equine, Gallus, Porcine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human AMPK alpha 1 (351-450/559aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW64 kDa
TargetPRKAA1
RRIDAB_10746294
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesAMPK alpha-1; 5 AMP activated protein kinase alpha
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NoteFor research use only
Expiration Date12 months from date of receipt.
AMPK alpha 1 Rabbit Polyclonal Antibody

Blank control (Black line): Mouse spleen (Black). Primary Antibody (green line): Rabbit Anti-AMPK alpha1 antibody (orb10076), Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 10000 events was performed.

AMPK alpha 1 Rabbit Polyclonal Antibody

Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-AMPK alpha 1 antibody (orb10076), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

AMPK alpha 1 Rabbit Polyclonal Antibody

Blank control: U937. Primary Antibody (green line): Rabbit Anti-AMPK alpha 1 antibody (orb10076), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

AMPK alpha 1 Rabbit Polyclonal Antibody

Sample: Hela (Human) Cell Lysate at 40 ug, Primary: Anti-AMPK alpha 1 (orb10076) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64 kD, Observed band size: 64 kD.

AMPK alpha 1 Rabbit Polyclonal Antibody

Sample: HepG2 (Human) Cell Lysate at 40 ug, Primary: Anti-AMPK alpha 1 (orb10076) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64 kD, Observed band size: 64 kD.

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