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Anti-HLA-E Purified
Catalog Number: orb44421
| Catalog Number | orb44421 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to HLA-E. |
| Clonality | Monoclonal |
| Clone Number | MEM-E/02 |
| Tested applications | IHC-P, WB |
| Reactivity | Human, Primate |
| Isotype | Mouse IgG1 |
| Immunogen | Recombinant human HLA-E denaturated heavy chain. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | HLA-E |
| Entrez | 3133 |
| UniProt ID | P13747 |
| RRID | AB_10998682 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-HLA-E antibody Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: human placenta; heat antigen retrieval (sodium citrate), incubation with mAb: 1 hour / RT, detection DAB. |
| Expiration Date | 12 months from date of receipt. |
Anti-Hu HLA-E purified antibody (clone MEM-E/02) specificity verification by flow cytometry Anti-Hu HLA-E purified antibody (concentration in sample 5 μg/ml, red-filled histogram) binds specifically to surface HLA-E on surface of HLA-E transfected LCL 721.221 cells, but not to RAJI cells, K562 cells, HLA-G transfected LCL 721.221 cells and non-transfected LCL 721.221 cells. Level of non-specific binding was assessed using Mouse IgG1 isotype control PE (MOPC-21) under same conditions (concentration in sample 5 μg/ml, black-dashed histogram).
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