HLA E Rabbit Polyclonal Antibody

• Catalog Number: orb10825
• Category: Antibodies
• Description: HLA E Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HLA E (251-358/358aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=3ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: HLA-E
• UniProt ID: P13747
• RRID: AB_10752629
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HLA class I histocompatibility antigen E alpha cha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-HLAE antibody (orb10825), Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 3 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: K562 Cell (Human) Lysate at 30 ug, Primary: Anti-HLA E at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 35 kD.

Sample: Raji Cell (Human) Lysate at 30 ug, Primary: Anti-HLA E at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 35 kD.