Anti-HLA-E Purified

• Catalog Number: orb44422
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-E.
• Clonality: Monoclonal
• Clone Number: MEM-E/06
• Tested applications: FC, IHC-P, IP
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA-E
• Entrez: 3133
• UniProt ID: P13747
• RRID: AB_10991554
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-E antibody
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from HLA-E negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-HLA-E (MEM-E/06) purified antibody (concentration in sample 0.56 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-HLA-E (MEM-E/06) purified antibody (concentration in sample 0.56 µg/ml, GAM APC).

Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/06).