HLA-E Antibody

SKU: orb44423

Description

Mouse Monoclonal to HLA-E.

Research Area

Epigenetics, Immunology, Infectious Diseases

Images & Validation

• Tested Applications: FC, IHC-P, IP
• Reactivity: Human
• Application Notes:
  Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen. Flow cytometry: Recommended dilution: 1-4 µg/ml

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: MEM-E/07
• Immunogen: Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
• Target: HLA-E
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E

Separation of HLA-E transfected LCL cells stained using anti-human HLA-E (MEM-E/07) purified antibody (concentration in sample 2 µg/ml, red-filled) from HLA-E transfected LCL cells stained using mouse IgG1 isotype control (MOPC-21) purified antibody (concentration in sample 2 µg/ml, same as HLA-E purified antibody concentration, black-dashed) in flow cytometry analysis (surface staining).

Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/07).

Quick Database Links

Gene Symbol

HLA-E

UniProt

P13747

NCBI Gene ID

3133