Anti-HLA Class I Purified Low Endotoxin

• Catalog Number: orb179914
• Category: Antibodies
• Description: Mouse monoclonal antibody to HLA-A
• Clonality: Monoclonal
• Clone Number: W6/32
• Tested applications: ELISA, FA, FC, ICC, IHC-Fr, IP
• Reactivity: Bovine, Feline, Human, Primate
• Isotype: Mouse IgG2a
• Immunogen: Membrane of human tonsil cells
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: Functional application: The antibody W6/32 is suitable as a positive control for HLA tissue typing. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA Class I
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-HLA A Antibody, anti-HLA B Antibody, anti-HLA
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Separation of leukocytes stained using anti-HLA Class I (W6/32) purified antibody (concentration in sample 4 μg/ml, GAM APC, red-filled) from leukocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-HLA Class I (W6/32) purified antibody (concentration in sample 4 μg/ml, GAM APC).