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HLA-C Mouse Monoclonal Antibody

Catalog Number: orb1607474

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1607474
CategoryAntibodies
DescriptionHLA-C Mouse Monoclonal Antibody
TargetHLA-C
ClonalityMonoclonal
Species/HostMouse
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenRecombinant human HLA-C protein (25-305/366aa)
UniProt IDP10321
MW4 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500, Flow-Cyt=1ug/Test
Antibody TypePrimary Antibody
Clone Number3C1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesHLA-C; Major Histocompatibility Complex, Class I;
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NoteFor research use only
HLA-C Mouse Monoclonal Antibody

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Recombinant human HLA-C & Beta-2-MG Heterodimer protein, C-His (HEK293), Primary: Anti-HLA-C (orb1607474) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 41 kDa, Observed band size: 45 kDa.

HLA-C Mouse Monoclonal Antibody

Blank control: U266. Primary Antibody (green line): Mouse Anti-HLA-C antibody (orb1607474), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HLA-C Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HLA-C) Monoclonal Antibody, Unconjugated (orb1607474) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

HLA-C Mouse Monoclonal Antibody

Blank control: U266. Primary Antibody (green line): Mouse Anti-HLA-C antibody (orb1607474), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HLA-C Mouse Monoclonal Antibody

Blank control: U266. Primary Antibody (green line): Mouse Anti-HLA-C antibody (orb1607474), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HLA-C Mouse Monoclonal Antibody

Blank control: U266. Primary Antibody (green line): Mouse Anti-HLA-C antibody (orb1607474), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HLA-C Mouse Monoclonal Antibody

Blank control: U266. Primary Antibody (green line): Mouse Anti-HLA-C antibody (orb1607474), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HLA-C Mouse Monoclonal Antibody

Blank control: U266. Primary Antibody (green line): Mouse Anti-HLA-C antibody (orb1607474), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HLA-C Mouse Monoclonal Antibody

Blank control: U266. Primary Antibody (green line): Mouse Anti-HLA-C antibody (orb1607474), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-MouseIgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mousse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HLA-C Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HLA-C) Monoclonal Antibody, Unconjugated (orb1607474) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

HLA-C Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HLA-C) Monoclonal Antibody, Unconjugated (orb1607474) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

HLA-C Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HLA-C) Monoclonal Antibody, Unconjugated (orb1607474) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

HLA-C Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HLA-C) Monoclonal Antibody, Unconjugated (orb1607474) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

HLA-C Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HLA-C) Monoclonal Antibody, Unconjugated (orb1607474) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

HLA-C Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HLA-C) Monoclonal Antibody, Unconjugated (orb1607474) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

HLA-C Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HLA-C) Monoclonal Antibody, Unconjugated (orb1607474) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

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    FC,  IP,  WB

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    ELISA,  FA,  FC,  ICC,  IHC-Fr,  IP

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    ELISA,  FC,  ICC,  IHC-Fr,  IP

    Bovine, Feline, Human, Primate

    Monoclonal

    Unconjugated

    0.1 mg