HLA-B Antibody (N-term)

• Catalog Number: orb1927191
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This HLA-B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 75-107 amino acids from the N-terminal region of human HLA-B.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P30464
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25
• Clone Number: RB47637
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: HLA class I histocompatibility antigen, B-15 alpha
• Note: For research use only

Immunohistochemical analysis of paraffin-embedded H. spleen section using HLA-B Antibody (N-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. tonsil section using HLA-B Antibody (N-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded R. spleen section using HLA-B Antibody (N-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

All lanes: Anti-HLA-B Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HL-60 whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-HLA-B Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HL-60 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: Ramos whole cell lysate. Lane 5: human spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining HLA-B in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.