Anti-GSK3 alpha/beta Antibody

• Catalog Number: orb214019
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GSK3A
• Target: GSK3A; GSK3B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat, Zebrafish
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GSK3 alpha/beta. The exact sequence is proprietary.
• UniProt ID: P18266, P49841, Q9WV60, P49840, Q2NL51, P18265
• Tested applications: IH, WB
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti GSK3A antibody, anti Glycogen synthase kinase
• Note: For research use only
• Entrez: 50686, 56637, 2931, 84027, 606496

Western blot analysis of GSK3 alpha/beta expression in 293T (A), A549 (B), Hela (C), mouse brain (D), rat brain (E) whole cell lysates. (Predicted band size: 50; 46 kD; Observed band size: 51; 46 kD)

Immunohistochemical analysis of GSK3 alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.