Anti-GSK3 alpha/beta (Phospho-Y279/216) Antibody

• Catalog Number: orb216120
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GSK3 alpha/beta (Phospho-Y279/216)
• Target: GSK3A; GSK3B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat, Zebrafish
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y279/216 of human GSK3 alpha/beta protein. The exact sequence is proprietary.
• UniProt ID: P18266, P49841, Q9WV60, P49840, Q2NL51, P18265
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti GSK3A antibody, anti Glycogen synthase kinase
• Note: For research use only
• Entrez: 50686, 56637, 2931, 84027, 606496

Western blot analysis of GSK3 alpha/beta (Phospho-Y279/216) expression in mouse testis (A), rat testis (B), rat spleen (C) whole cell lysates. (Predicted band size: 50; 46 kD; Observed band size: 51; 46 kD)

Immunohistochemical analysis of GSK3 alpha/beta (Phospho-Y279/216) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.