Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Rabbit Polyclonal Antibody

SKU: orb783420

Description

Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Tyr216 VS(p-Y)IC
• Target: GSK3B
• Molecular Weight: 47/51 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GSK3 alpha & beta (p-Y279/Y216); p-GSK3 alpha & beta; phospho-GSK3 alpha & beta; 7330414F15Rik; 8430431H08Rik; GSK-3; GSK-3beta; GSK3; GSK3B_HUMAN; GSK3B; GSK-3 beta; Serine/threonine-protein kinase GSK3B; 2.7.11.26; GSK3B_MOUSE;

Positive control: RSC96, Isotype Control Antibody: Rabbit IgG, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody catalog number: orb783420, dilution: 3 µl in 100 µl 1X PBS containing 0.5% BSA.

Sample: Lane 1: Brain (Mouse) Lysate at 30 ug, Lane 2: Testis (Mouse) Lysate at 30 ug, Primary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (orb783420) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 47/51 kD, Observed band size: 50 kD.

Sample: Lane 1: Muscle (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Mouse) Lysate at 40 ug, Lane 4: Pancreas (Mouse) Lysate at 40 ug, Lane 5: Thymus (Mouse) Lysate at 40 ug, Lane 6: Muscle (Rat) Lysate at 40 ug, Lane 7: Liver (Rat) Lysate at 40 ug, Lane 8: Cerebrum (Rat) Lysate at 40 ug, Lane 9: Pancreas (Rat) Lysate at 40 ug, Lane 10: Thymus (Rat) Lysate at 40 ug, Lane 11: MCF-7 (Human) Cell Lysate at 30 ug, Lane 12: A431 (Human) Cell Lysate at 30 ug, Lane 13: U2os (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (orb783420) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47'51 kD, Observed band size: 47 kD.

Quick Database Links

Gene Symbol

GSK3B

UniProt

P49841