Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Rabbit Polyclonal Antibody

• Catalog Number: orb783420
• Category: Antibodies
• Description: Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Tyr216 VS(p-Y)IC
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 47/51 kDa
• Target: GSK3B
• UniProt ID: P49841
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: GSK3B(phospho-Tyr216); GSK3B(phospho-Y216); p-GSK-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Positive control: RSC96, Isotype Control Antibody: Rabbit IgG, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody catalog number: orb783420, dilution: 3 µl in 100 µl 1X PBS containing 0.5% BSA.

Sample: Lane 1: Brain (Mouse) Lysate at 30 ug, Lane 2: Testis (Mouse) Lysate at 30 ug, Primary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (orb783420) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 47/51 kD, Observed band size: 50 kD.

Sample: Lane 1: Muscle (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Mouse) Lysate at 40 ug, Lane 4: Pancreas (Mouse) Lysate at 40 ug, Lane 5: Thymus (Mouse) Lysate at 40 ug, Lane 6: Muscle (Rat) Lysate at 40 ug, Lane 7: Liver (Rat) Lysate at 40 ug, Lane 8: Cerebrum (Rat) Lysate at 40 ug, Lane 9: Pancreas (Rat) Lysate at 40 ug, Lane 10: Thymus (Rat) Lysate at 40 ug, Lane 11: MCF-7 (Human) Cell Lysate at 30 ug, Lane 12: A431 (Human) Cell Lysate at 30 ug, Lane 13: U2os (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (orb783420) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47'51 kD, Observed band size: 47 kD.