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Item 1 of 4
Item 1 of 4
| Catalog Number | orb764196 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to GSK3alpha/beta (phospho-Tyr279/216) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GSK3 alpha/beta around the phosphorylation site of Tyr279/216. AA range:246-295 |
| Concentration | 1 mg/ml |
| Dilution range | IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 51,46 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-GSK3A antibody, anti-Glycogen synthase kinase Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of Hela cell. 1, GSK3α/β (phospho Tyr279/216) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
Immunofluorescence analysis of mouse-liver tissue. 1, GSK3α/β (phospho Tyr279/216) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunofluorescence analysis of mouse-liver tissue. 1, GSK3α/β (phospho Tyr279/216) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Western Blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800 (diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute? Plasma Membrane Protein Isolation and Cell Fractionation Kit (SM-005, Inventbiotech, MN, USA).
