Anti-gp91 phox Antibody

• Catalog Number: orb213813
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CYBB
• Target: CYBB
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human gp91 phox. The exact sequence is proprietary.
• UniProt ID: P04839, Q61093
• Tested applications: IH, WB
• Dilution range: WB: 1-500-1-1000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti NOX2 antibody, anti Cytochrome b-245 heavy ch
• Note: For research use only
• Entrez: 13058, 1536

Western blot analysis of gp91 phox expression in Jurkat (A), Beas2B (B) whole cell lysates. (Predicted band size: 65 kD; Observed band size: 50; 91 kD)

Immunohistochemical analysis of gp91 phox staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.