NOX2 Rabbit Polyclonal Antibody

• Catalog Number: orb6523
• Category: Antibodies
• Description: NOX2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NOX2 (501-570/570aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: CYBB
• UniProt ID: P04839
• RRID: AB_10928664
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AMCBX2; CGD; CGD91-phox; CY24B_HUMAN; CYBB; Cytoch
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ye, Zhen-Nan et al. Inhibition of leukotriene B4 synthesis protects against early brain injury possibly via reducing the neutrophil-generated inflammatory response and oxidative stress after subarachnoid hemorrhage in rats Behav Brain Res, 339, 19-27 (2018)

PubMed: 29133197

Sample: Lane 1: Rat Bronchus tissue lysates, Lane 2: Rat Esophagus tissue lysates, Lane 3: Human HL60 cell lysates, Primary: Anti-NOX2 (orb6523) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kDa, Observed band size: 65 kDa.

Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-NOX2 (orb6523) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-NOX2 (orb6523) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NOX2 Polyclonal Antibody, Unconjugated (orb6523) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.