Glial Fibrillary Acidic Proetin (GFAP) Antibody

SKU: orb319549

Description

Our Anti-Glial Fibrillary Acidic Protein (GFAP) primary antibody from Biorbyt is rabbit polyclonal. It detects bovine, horse, human, mouse, and rat Glial Fibrillary Acidic Proetin (GFAP) and is neat serum.

Research Area

Disease Biomarkers, Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 1:1000-1:5000, IHC: 1:300-1:5000, ICC: 1:1000-1:5000
• Reactivity: Bovine, Equine, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human GFAP.
• Target: Glial Fibrillary Acidic Proetin (GFAP)
• Molecular Weight: 50
• Purification: Neat Serum
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Neat serum
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

wu:fb34h11 antibody, ALXDRD antibody, cb345 antibody, etID36982.3 antibody, FLJ42474 antibody, FLJ45472 antibody, GFAP antibody, GFAP_HUMAN antibody, gfapl antibody, Glial fibrillary acidic protein antibody, Intermediate filament protein antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody

Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (Cat. orb319549, 1:5000,green), colabeled with anti-calbindin (Cat. orb378472 , 1:2,000, red), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.

Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (Cat. orb319549, 1:5000,green), colabeled with anti-MeCP2 (Cat. orb55908, 1:500, red), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.

Immunofluorescence of a section of rat cerebellum labeled with anti-visinin-like 1 protein (Cat. orb319709, 1:500, red) and anti-GFAP (Cat. orb319549, green, 1:5000). The anti-VSNL1 revels protein expressed in the granule cell menbranes and in synapses in the white matter and molecular layers. The anti-GFAP reveals protein expressed in the processes of Bergmann glia and astroglia.

Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (Cat. orb319585, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP (Cat. orb319549, 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.

Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (Cat. orb319549, 1:1000, green) and anti-MAP2 (orb319566, 1:1000, red).

Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP (Cat. orb319549, 1:1000, green) and anti-MAP2 (orb319566, 1:1000, red).

Mixed neuron/glial cultures stained with anti-vimentin (green, 1:500) and rabbit anti-GFAP antibody (Cat. orb319549, red, 1:1000). The blue stains nuclear DNA. Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).

Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.

Quick Database Links

Gene Symbol

Glial Fibrillary Acidic Proetin (GFAP)

UniProt

P14136