GCTM-2 Mouse Monoclonal Antibody

SKU: orb1474172

Description

The GCTM-2 Antibody is suitable for IF, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GCTM-2. The exact sequence is proprietary.

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB (1/1000 - 1/5000), IF/IC (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GCTM-2. The exact sequence is proprietary.
• Target: PODXL
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: crude ascites with 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PCLP; PCLP1; Podocalyxin; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1

Western blot analysis of GCTM-2 expression in A549 (A) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 58 kD)

Immunofluorescent analysis of GCTM-2 staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

PODXL

UniProt

O00592

NCBI Gene ID

5420