PODXL Antibody (C-term) (Ascites)

SKU: orb652178

Description

Mouse monoclonal antibody to PODXL

Research Area

Cancer Biology, Cardiovascular Research, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:100
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Immunogen: Synthetic Peptide
• Target: PODXL
• Molecular Weight: 58635
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Podocalyxin antibody, anti-GCTM-2 antigen antibody, anti-Gp200 antibody, anti-Podocalyxin-like protein 1 antibody, anti-PC antibody, anti-PCLP-1 antibody, anti-PODXL antibody, anti-PCLP antibody, anti-PCLP1 antibody

Anti-PODXL Antibody (C-term) (Ascites) at 1:16000 dilution + A549 whole cell lysate. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58635 Da. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of SY5Y cells stained with PODXL (C-term) Antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with PODXL (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Quick Database Links

Gene Symbol

PODXL

UniProt

O00592