PODXL Antibody

SKU: orb388565

Description

Mouse monoclonal antibody to PODXL

Research Area

Cardiovascular Research, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: FACS: 0.5-1 μg/million cells, IF/ICC: 1-2 μg/ml, IP: 1-2 μg/500 μg protein, WB: 0.5-1 μg/ml, IHC-P: 0.5-1 μg/ml
• Reactivity: Human, Rabbit, Rat
• Application Notes:
  Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker)

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 3D3
• Immunogen: A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin
• Target: PODXL
• Source/Expression System: Bioreactor
• Molecular Weight: 165-170kDa
• Purification: Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx; Podocalyxin-like protein 1

Flow cytometric analysis of NCCIT Cells using PODXL antibody

Immunohistochemical staining of human angiosarcoma tissue using PODXL antibody

Immunohistochemical staining of human Placenta tissue using PODXL antibody

Western blot analysis of Hela Lysate using PODXL antibody

Quick Database Links

Gene Symbol

PODXL

UniProt

O00592