Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Antibody

• Catalog Number: orb388565
• Category: Antibodies
• Description: Mouse monoclonal antibody to PODXL
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3D3
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Rabbit, Rat
• Isotype: IgG1
• Immunogen: A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: FACS: 0.5-1 μg/million cells, IF/ICC: 1-2 μg/ml, IP: 1-2 μg/500 μg protein, WB: 0.5-1 μg/ml, IHC-P: 0.5-1 μg/ml
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 165-170kDa
• Target: PODXL
• Entrez: 5420
• UniProt ID: O00592
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx; Podocalyxi
• Note: For research use only
• Application notes: Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker)
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of NCCIT Cells using PODXL antibody

Immunohistochemical staining of human angiosarcoma tissue using PODXL antibody

Immunohistochemical staining of human Placenta tissue using PODXL antibody

Western blot analysis of Hela Lysate using PODXL antibody