Anti-CD28 antibody(DM63); Rabbit mAb

• Catalog Number: orb757386
• Category: Antibodies
• Description: Anti-CD28 antibody(DM63), Rabbit mAb
• Target: CD28
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P10747
• Tested applications: ELISA, FC
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DM63
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD28, Tp44
• Note: For research use only

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD28 protein, mFc-His tagged protein orb689375 can bind Rabbit anti-CD28 monoclonal antibody (clone: DM63) in a linear range of 1-100 ng/ml.

Expi 293 cell line transfected with irrelevant protein (A) and human CD28 (B) were surface stained with Rabbit anti-CD28 monoclonal antibody 1 μg/ml (clone: DM63) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry data of serially titrated Rabbit anti-CD28 monoclonal antibody (clone: DM63) on Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Flow cytometry analysis of antigen binding of rabbit anti-human CD28 mAb (orb757386). (A) orb757386 does not bind to 293T cells that do not express CD28. (B) A clear peak shift of orb757386 was seen compared to the control when incubated with CD28-expressing Jurkat cells, indicating strong binding of orb757386 to CD28. Antibodies were incubated at 2 μg/mL.