CD133 Antibody

• Catalog Number: orb339105
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PROM1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD133. The exact sequence is proprietary.
• UniProt ID: O43490, O54990
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PROML1; Prominin-1; Antigen AC133; Prominin-like p
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 8842, 19126
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD133 expression in K562 (A), SW620 (B), Jurkat (C), mouse kidney (D), mouse lung (E) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 120 kD)

Immunohistochemical analysis of CD133 staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.