Anti-CD133 Antibody

• Catalog Number: orb339105
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PROM1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD133. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PROM1
• Entrez: 8842, 19126
• UniProt ID: O43490, O54990
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti PROML1 antibody, anti Prominin-1 antibody, an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD133 expression in K562 (A), SW620 (B), Jurkat (C), mouse kidney (D), mouse lung (E) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 120 kD)

Immunohistochemical analysis of CD133 staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.