CD133/Prominin-1 (Intracellular domain) Antibody

SKU: orb1294396

Description

Rabbit polyclonal antibody to CD133

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:300-1:500 Immunohistochemistry (Paraffin) 1:200-1:400
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human CD133.
• Target: CD133 / Prominin-1 (Intracellular domain)

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

All lanes: Anti-CD133 antibody at 1/700 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294396 overnight at 4°C.

All lanes: Anti-CD133 antibody at 1/700 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294396 overnight at 4°C.

All lanes: Anti-CD133 antibody at 1/700 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294396 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294396 anti-CD133 / Prominin-1 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD133 / Prominin-1 with orb1294396 at 1/200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD133 / Prominin-1 with orb1294396 at 1/200.

Quick Database Links

Gene Symbol

CD133 / Prominin-1 (Intracellular domain)