CD133 Rabbit Polyclonal Antibody

SKU: orb526485

Description

CD133 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD133 (508-552/865aa)
• Target: PROM1
• Molecular Weight: 95 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

AC133; Antigen AC133; Hematopoietic stem cell antigen; hProminin; PROM1; Prominin I; Prominin 1; Prominin1; Prominin-1; Prominin like protein 1 precursor; Prominin mouse like 1; prominin1; PROML1; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4.

Sample: SP2/0 Cell (Mouse) Lysate at 40 ug, Colon carcinoma (Human) Lysate at 40 ug, Primary: Anti-CD133 (orb526485) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 95 kD, Observed band size: 95/120 kD.

Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-CD133 antibody (orb526485), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: SP2/0 (Mouse) Cell Lysate at 30 ug Lane 2: Lovo (Human) Cell Lysate at 30 ug Lane 3: SW480 (Human) Cell Lysate at 30 ug Lane 4: Uterus (Mouse) Lysate at 40 ug Primary: Anti-CD133 (orb526485) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kD Observed band size: 115 kD.

The blue histogram is unstained cells (HepG 2). The Orange histogram is cells stained with Rabbit IgG/FITC (orb525556), The green histogram is cells stained with Rabbit Anti-CD133/FITC Conjugated antibody. Isotype control: Cell lines treated with Rabbit IgG/FITC (orb525556) instead of the primary antibody to confirm that primary antibody binding is 2 µg/5 µg/1 µg in 100 µl 1X PBS containing 0.5% BSA.

The blue histogram is unstained cells (HepG 2). The Orange histogram is cells stained with Rabbit IgG/PE, The green histogram is cells stained with Rabbit Anti-CD133/PE Conjugated antibody. Isotype control: Cell lines treated with Rabbit IgG/PE instead of the primary antibody to confirm that primary antibody binding is specific. 2 µg/5 µg/10 µg in 100 µl 1X PBS containing 0.5% BSA.

Quick Database Links

Gene Symbol

PROM1

UniProt

O43490

References

Leticia Elizabeth Augustin Czeczko 1 2, Carmen Australia Paredes Marcondes Ribas 1 2, Nicolau Gregori Czeczko 1 2, Thelma Larocca Skare 1, Camila Kienen Yamakawa 1, Guilherme Gionedis 1, Cecilia Vasconcelos 1, Fabiola Pabst Bremer 1 2, Diogo Francesco Castoldi 1 2, Martin Gasser 1, Ana Maria Waaga-Gasser ARE STEM CELL MARKER EXPRESSION AND CD133 ANALYSIS RELEVANT TO DIFFERENTIATE COLORECTAL CANCER? Arq Bras Cir Dig, (2025)

PubMed: 34669880