CD133 antibody

SKU: orb99113

Description

CD133 antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Dilution range: IHC-P:1:200, WB: 1:500, IF/ICC: 1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD133. Please contact us for the exact immunogen sequence. The peptide is available as orb374954.
• Target: CD133. Human;Mouse;Rat. No cross reactivity with other proteins.
• Molecular Weight: 95 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide, 10 mg/ml rAlbumin
• Concentration: - 100 μg (in 100 μl): 1 mg/ml- 200 μg (in 200 μl): 1 mg/ml
• Disclaimer: For research use only

Alternative Names

AC133 antibody,gen AC133 antibody, CD133 antibody, CD133 Partial Recombinant Protein antibody, CORD12 antibody, Hematopoietic stem cellgen antibody, hProminin antibody, MCDR2 antibody, MSTP061 antibody, PROM antibody, PROM1 antibody, Prominin I antibody, Prominin like protein 1 precursor antibody, Prominin mouse like 1 antibody, prominin1 antibody, PROML1 antibody, RP41 antibody, STGD4 antibody, AC133 antibody, Antigen AC133 antibody, Hematopoietic stem cell antigen antibody, hProminin antibody, PROM1 antibody, Prominin I antibody, Prominin like protein 1 precursor antibody, Prominin mouse like 1 antibody, prominin1 antibody, PROML1 antibody, CD133 antibody, CORD12 antibody, MCDR2 antibody, MSTP061 antibody, PROML1 antibody, RP41 antibody, STGD4.

Immunohistochemical analysis of paraffin-embedded human mammary cancer tissue using CD133 antibody

IHC-P analysis of mouse heart tissue using CD133 antibody (Primary antibody diluted to 1:100)

Western blot analysis of human cancer cell lysates using CD133 antibody [Reviews]

WB analysis of rat small intestine tissue using CD133 antibody (dilution at 1:200-1:1000 based on 1mg/mL)

Western Blot on human cells using CD133 antibody (Protocol steps under Review A) [Reviews]

IF analysis analysis of mouse lung tissue using CD133 antibody (Dilution of primary antibody 1:200)

IHC-P analysis of mouse liver tissue using CD133 antibody (Dilution of primary antibody 1:100)

IHC-P analysis of mouse liver tissue using CD133 antibody (Primary antibody at 1:100)

Immunohistochemical staining of paraffin embedded human breast cancer tissue using anti-CD206 (primary antibody at 1:200)

IHC-P analysis of mouse heart tissue using CD133 antibody (Primary antibody at 1:100)

IHC-P analysis of mouse brain tissue using CD133 antibody (Dilution of primary antibody 1:100)

Immunofluorescent analysis of mouse brain tissue using CD133 antibody (Primary antibody diluted to 1:200)

Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse skin tissue using CD133 antibody (Dilution of primary antibody 1:100)

IHC-P analysis of human lung cancer tissue using CD133 antibody (Dilution of primary antibody 1:100)

Immunohistochemical analysis of formalin-fixed and paraffin embedded human muscle tissue using CD133 antibody (Primary antibody diluted to 1:200)

IHC-P analysis of human colonic adenocarcinoma tissue using CD133 antibody (Primary antibody at 1:100)

IHC-P analysis of human muscle tissue using CD133 antibody (Primary antibody at 1:100)

Immunohistochemical analysis of formalin-fixed and paraffin embedded human colonic adenocarcinoma tissue using CD133 antibody (Dilution of primary antibody 1:100)

IHC-P analysis of human lung cancer tissue using CD133 antibody (Primary antibody diluted to 1:100)

IHC-P analysis of human muscle tissue using CD133 antibody (Primary antibody diluted to 1:100)

IHC-P analysis of human colonic adenocarcinoma tissue using CD133 antibody (Primary antibody at 1:100)

Western blot analysis of human muscle (Lane1), Rat muscle (Lane2), Rat bladder (Lane3) using CD133 antibody (Dilution at: 1:500)

Western blot analysis of rat small intestine tissue using CD133 antibody (dilution at 1:200-1:1000 based on 1mg/mL)

Immunohistochemical staining of human mammary fibroma tissue using anti-CD206 (dilution of primary antibody - 1:100)

IHC-P image of human breast cancer tissue using anti-CD206 (dilution of primary antibody at 1:100)

Western blot analysis of Mouse large intestine (Lane 1), Mouse spinal cord (Lane 2) using CD133 antibody (Dilution of primary antibody 1:200)

Immunohistochemical staining of paraffin embedded human endometrial cancer tissue using anti-CD206 (primary antibody at 1:200)

IHC-P image of human endometrial cancer tissue using anti-CD206 (dilution of primary antibody at 1:100)

IF analysis of mouse skin tissue using CD133 antibody (Dilution of primary antibody 1:200)

IHC-P analysis of mouse kidney tissue using CD133 antibody (Primary antibody diluted to 1:200)

Quick Database Links

Gene Symbol

CD133. Human;Mouse;Rat. No cross reactivity with other proteins.

UniProt

O54990, O43490

NCBI Gene ID

8842

RefSeq:001139319, 001145847

References

G Faa, G Pichiri, P Coni, A Dessì, M Fraschini, V Fanos They will be famous: Multipotent stem cells in breast milk World Journal of Clinical Pediatrics, (2025)

PubMed:

Anna Lis-Nawara, Patrycja Kulis, Adrianna Łukawska, Piotr Ziółkowski, Marek Murawski, Julia Bar Pattern of the CD133 and sonic hedgehog protein (SHH) components of the sonic hedgehog signaling pathway expression and their correlation with ovarian and colorectal cancer parameters journals.viamedica.pl, (2025)

PubMed:

Applications

IHC

Reactivity

Human

Feng, Long et al. Isolation and phenotypic characterization of cancer stem-like side population cells in colon cancer Mol Med Rep, 12, 3531-3536 (2015)

PubMed: 25997915

Applications

ICC

Reactivity

Human

Winardi, Daniel et al. Correlation of altered expression of the autophagy marker LC3B with poor prognosis in astrocytoma Biomed Res Int, 2014, 723176 (2014)

PubMed: 24900981

Chen, Jintao et al. Proteomic Distributions in CD34+ Microvascular Niche Patterns of Glioblastoma J Histochem Cytochem, (2021)

PubMed: 34751042

Applications

IF

Feitosa, Neli Patrícia Pereira et al. Cancerous and non-neoplastic stem cells in the stomach similarly express CD44 and CD133 Acta Histochem, 123, 151787 (2021)

PubMed: 34517259

Kase, Marju et al. Impact of CD133 positive stem cell proportion on survival in patients with glioblastoma multiforme Radiol Oncol, 47, 405-410 (2013)

PubMed: 24294187

Hirashima, Kanji et al. Cell biological profiling of reprogrammed cancer stem cell-like colon cancer cells maintained in culture Cell Tissue Res, 375, 697-707 (2019)

PubMed: 30284085

Nowacki, Maciej et al. Nanovehicles as a novel target strategy for hyperthermic intraperitoneal chemotherapy: a multidisciplinary study of peritoneal carcinomatosis Oncotarget, 6, 22776-22798 (2015)

PubMed: 26254295

Zou, Yuhong et al. Nrf2 is involved in maintaining hepatocyte identity during liver regeneration PLoS One, 9, e107423 (2014)

PubMed: 25222179

Coni, Pierpaolo et al. Exploring cell surface markers and cell-cell interactions of human breast milk stem cells J Public Health Res, 12, (2023)

PubMed: 36712902

Yang, Meng-Yin et al. An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior J Biomed Mater Res B Appl Biomater, 103, 618-628 (2015)

PubMed: 24966152

Zhu, Yifan et al. Influence of interferon-α on the expression of the cancer stem cell markers in pancreatic carcinoma cells Exp Cell Res, 324, 146-156 (2014)

PubMed: 24726912

Nadal, Rosa et al. CD133 expression in circulating tumor cells from breast cancer patients: potential role in resistance to chemotherapy Int J Cancer, 133, 2398-2407 (2013)

PubMed: 23661576

Tu, Zhenbo et al. CXCR4 is involved in CD133-induced EMT in non-small cell lung cancer Int J Oncol, 50, 505-514 (2017)

PubMed: 28000861

Applications

IHC

Reactivity

Human

Sun, Zhonghai et al. Tumour stem cell markers CD133 and CD44 are useful prognostic factors after surgical resection of pancreatic neuroendocrine tumours Oncol Lett, 20, 341 (2020)

PubMed: 33123252

Applications

IHC

Reactivity

Human

Jensen, Todd et al. Descriptive analysis of tumor cells with stem like phenotypes in metastatic and benign adrenal tumors J Pediatr Surg, 50, 1493-1501 (2015)

PubMed: 25976447