CD133 antibody

• Catalog Number: orb99113
• Category: Antibodies
• Description: CD133 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD133. Please contact us for the exact immunogen sequence. The peptide is available as orb374954.
• Concentration: - 100 μg (in 100 μl): 1 mg/ml; - 200 μg (in 200 μl): 1 mg/ml
• Dilution range: IHC-P:1:200, WB: 1:500, IF/ICC: 1:200
• Form/Appearance: 10 mM PBS, 0.02% sodium azide, 10 mg/ml rAlbumin
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 95 kDa
• Target: CD133. Human;Mouse;Rat. No cross reactivity with other proteins.
• Entrez: 8842
• UniProt ID: O43490, O54990
• NCBI: 001145847, 001139319
• Storage: After reconstitution store at 4°C.for up to two weeks. For long term storage, aliquot and store at -20°C. Avoid freeze/thaw cycles.
• Alternative names: AC133 antibody,gen AC133 antibody, CD133 antibody,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Feng, Long et al. Isolation and phenotypic characterization of cancer stem-like side population cells in colon cancer Mol Med Rep, 12, 3531-3536 (2015)

PubMed: 25997915

Winardi, Daniel et al. Correlation of altered expression of the autophagy marker LC3B with poor prognosis in astrocytoma Biomed Res Int, 2014, 723176 (2014)

PubMed: 24900981

Chen, Jintao et al. Proteomic Distributions in CD34+ Microvascular Niche Patterns of Glioblastoma J Histochem Cytochem, (2021)

PubMed: 34751042

Feitosa, Neli Patrícia Pereira et al. Cancerous and non-neoplastic stem cells in the stomach similarly express CD44 and CD133 Acta Histochem, 123, 151787 (2021)

PubMed: 34517259

Kase, Marju et al. Impact of CD133 positive stem cell proportion on survival in patients with glioblastoma multiforme Radiol Oncol, 47, 405-410 (2013)

PubMed: 24294187

Hirashima, Kanji et al. Cell biological profiling of reprogrammed cancer stem cell-like colon cancer cells maintained in culture Cell Tissue Res, 375, 697-707 (2019)

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Nowacki, Maciej et al. Nanovehicles as a novel target strategy for hyperthermic intraperitoneal chemotherapy: a multidisciplinary study of peritoneal carcinomatosis Oncotarget, 6, 22776-22798 (2015)

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Zou, Yuhong et al. Nrf2 is involved in maintaining hepatocyte identity during liver regeneration PLoS One, 9, e107423 (2014)

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Coni, Pierpaolo et al. Exploring cell surface markers and cell-cell interactions of human breast milk stem cells J Public Health Res, 12, (2023)

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Yang, Meng-Yin et al. An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior J Biomed Mater Res B Appl Biomater, 103, 618-628 (2015)

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Zhu, Yifan et al. Influence of interferon-α on the expression of the cancer stem cell markers in pancreatic carcinoma cells Exp Cell Res, 324, 146-156 (2014)

PubMed: 24726912

Nadal, Rosa et al. CD133 expression in circulating tumor cells from breast cancer patients: potential role in resistance to chemotherapy Int J Cancer, 133, 2398-2407 (2013)

PubMed: 23661576

Tu, Zhenbo et al. CXCR4 is involved in CD133-induced EMT in non-small cell lung cancer Int J Oncol, 50, 505-514 (2017)

PubMed: 28000861

Nel, Ivonne et al. Circulating Tumor Cell Composition in Renal Cell Carcinoma PLoS One, 11, e0153018 (2016)

PubMed: 27101285

Sun, Zhonghai et al. Tumour stem cell markers CD133 and CD44 are useful prognostic factors after surgical resection of pancreatic neuroendocrine tumours Oncol Lett, 20, 341 (2020)

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Jensen, Todd et al. Descriptive analysis of tumor cells with stem like phenotypes in metastatic and benign adrenal tumors J Pediatr Surg, 50, 1493-1501 (2015)

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Liao, C. H. et al. Restoration of erectile function with intracavernous injections of endothelial progenitor cells after bilateral cavernous nerve injury in rats Andrology, 3, 924-932 (2015)

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Immunohistochemical analysis of paraffin-embedded human mammary cancer tissue using CD133 antibody

IHC-P analysis of mouse heart tissue using CD133 antibody (Primary antibody diluted to 1:100)

Western blot analysis of human cancer cell lysates using CD133 antibody [Reviews]

WB analysis of rat small intestine tissue using CD133 antibody (dilution at 1:200-1:1000 based on 1mg/mL)

Western Blot on human cells using CD133 antibody (Protocol steps under Review A) [Reviews]

IF analysis analysis of mouse lung tissue using CD133 antibody (Dilution of primary antibody 1:200)

IHC-P analysis of mouse liver tissue using CD133 antibody (Dilution of primary antibody 1:100)

IHC-P analysis of mouse liver tissue using CD133 antibody (Primary antibody at 1:100)

Immunohistochemical staining of paraffin embedded human breast cancer tissue using anti-CD206 (primary antibody at 1:200)

IHC-P analysis of mouse heart tissue using CD133 antibody (Primary antibody at 1:100)

IHC-P analysis of mouse brain tissue using CD133 antibody (Dilution of primary antibody 1:100)

Immunofluorescent analysis of mouse brain tissue using CD133 antibody (Primary antibody diluted to 1:200)

Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse skin tissue using CD133 antibody (Dilution of primary antibody 1:100)

IHC-P analysis of human lung cancer tissue using CD133 antibody (Dilution of primary antibody 1:100)

Immunohistochemical analysis of formalin-fixed and paraffin embedded human muscle tissue using CD133 antibody (Primary antibody diluted to 1:200)

IHC-P analysis of human colonic adenocarcinoma tissue using CD133 antibody (Primary antibody at 1:100)

IHC-P analysis of human muscle tissue using CD133 antibody (Primary antibody at 1:100)

Immunohistochemical analysis of formalin-fixed and paraffin embedded human colonic adenocarcinoma tissue using CD133 antibody (Dilution of primary antibody 1:100)

IHC-P analysis of human lung cancer tissue using CD133 antibody (Primary antibody diluted to 1:100)

IHC-P analysis of human muscle tissue using CD133 antibody (Primary antibody diluted to 1:100)

IHC-P analysis of human colonic adenocarcinoma tissue using CD133 antibody (Primary antibody at 1:100)

Western blot analysis of human muscle (Lane1), Rat muscle (Lane2), Rat bladder (Lane3) using CD133 antibody (Dilution at: 1:500)

Western blot analysis of rat small intestine tissue using CD133 antibody (dilution at 1:200-1:1000 based on 1mg/mL)

Immunohistochemical staining of human mammary fibroma tissue using anti-CD206 (dilution of primary antibody - 1:100)

IHC-P image of human breast cancer tissue using anti-CD206 (dilution of primary antibody at 1:100)

Western blot analysis of Mouse large intestine (Lane 1), Mouse spinal cord (Lane 2) using CD133 antibody (Dilution of primary antibody 1:200)

Immunohistochemical staining of paraffin embedded human endometrial cancer tissue using anti-CD206 (primary antibody at 1:200)

IHC-P image of human endometrial cancer tissue using anti-CD206 (dilution of primary antibody at 1:100)

IF analysis of mouse skin tissue using CD133 antibody (Dilution of primary antibody 1:200)

IHC-P analysis of mouse kidney tissue using CD133 antibody (Primary antibody diluted to 1:200)