Anti-c-RAF Antibody

• Catalog Number: orb323254
• Category: Antibodies
• Description: Rabbit polyclonal antibody to c-RAF
• Target: RAF1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant protein corresponding to human c-RAF.
• UniProt ID: P04049
• Tested applications: IH, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:50-1:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti RAF antibody, anti RAF proto-oncogene serine/
• Note: For research use only
• Entrez: 5894

Western blot analysis of c-RAF expression in Hela (A) whole cell lysates. (Predicted band size: 73 kD; Observed band size: 75 kD)

Immunohistochemical analysis of c-RAF staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.