Raf-1 (phospho Ser296) rabbit pAb

• Catalog Number: orb769856
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Raf-1 (phospho-Ser296)
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser296. AA range:271-320
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 74
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-RAF1 antibody, anti-RAF antibody, anti-RAF pr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of 293 cells using Phospho-Raf-1 (S296) Polyclonal Antibody diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using C-RAF (Phospho-Ser296) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using C-RAF (Phospho-Ser296) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using C-RAF (Phospho-Ser296) Antibody. The lane on the right is blocked with the phospho peptide.