Raf-1 (phospho Ser621) rabbit pAb Antibody

SKU: orb769857

Description

Rabbit polyclonal antibody to Raf-1 (phospho-Ser621)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser621. AA range:591-640
• Molecular Weight: 73
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-RAF1 antibody, anti-RAF antibody, anti-RAF proto-oncogene serine/threonine-protein kinase antibody, anti-Proto-oncogene c-RAF antibody, anti-cRaf antibody, anti-Raf-1 antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using C-RAF (Phospho-Ser621) Antibody.

Immunofluorescence analysis of HeLa cells, using C-RAF (Phospho-Ser621) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C-RAF (Phospho-Ser621) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with UV 5', using C-RAF (Phospho-Ser621) Antibody. The lane on the right is blocked with the phospho peptide.