Raf-1 (phospho Ser338) rabbit pAb Antibody

SKU: orb764318

Description

Rabbit polyclonal antibody to Raf-1 (phospho-Ser338)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser338. AA range:305-354
• Molecular Weight: 70
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-RAF1 antibody, anti-RAF antibody, anti-RAF proto-oncogene serine/threonine-protein kinase antibody, anti-Proto-oncogene c-RAF antibody, anti-cRaf antibody, anti-Raf-1 antibody

Western Blot analysis of various cells using Phospho-Raf-1 (S338) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using C-RAF (Phospho-Ser338) Antibody.

Western blot analysis of lysates from HeLa cells treated with paclitaxel 1uM 24h, using C-RAF (Phospho-Ser338) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).