Anti-c-RAF Antibody

• Catalog Number: orb214492
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RAF1
• Target: RAF1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-RAF. The exact sequence is proprietary.
• UniProt ID: P04049, P11345, Q99N57
• Tested applications: IF, WB
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti RAF antibody, anti RAF proto-oncogene serine/
• Note: For research use only
• Entrez: 24703, 110157, 5894

Western blot analysis of c-RAF expression in A431 (A), HeLa (B), RAW264.7 (C), C6 (D) whole cell lysates. (Predicted band size: 73 kD; Observed band size: 74 kD)

Immunofluorescent analysis of c-RAF staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.