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beta Actin Mouse Monoclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution Range | WB 1:3000-10000 IHC 1:100-1:200 ICC/IF 1:100-200 |
| Reactivity | Gallus, Human, Mouse, Rabbit, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 17A10 |
| Immunogen | A synthesized peptide derived from human beta Actin |
| Target | Actin, cytoplasmic 1 |
| Molecular Weight | 42 kDa |
| Purification | Affinity-chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Mouse IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required. |
| Concentration | 0.5mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of beta Actin expression in (1) HeLa cell lysate; (2) Rat brain lysate; (3) Mouse brain lysate; (4) Chicken lung lysate; (5) Rabbit testis lysate with beta Actin mouse mAb.

Immunohistochemical analysis of paraffin-embedded human heart, using beta Actin Mouse Monoclonal Antibody.

Immunofluorescent analysis of HepG2 cells, using beta Actin mAb.
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beta Actin Mouse Monoclonal Antibody (orb865754)
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