Alpha-fetoglobulin Mouse Monoclonal Antibody

SKU: orb1474099

Description

The Alpha-fetoglobulin Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Alpha-fetoglobulin. The exact sequence is proprietary.

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Alpha-fetoglobulin. The exact sequence is proprietary.
• Target: AFP
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

Western blot analysis of Alpha-fetoglobulin expression in human fetal liver (A) whole cell lysates. (Predicted band size: 68 kD; Observed band size: 72 kD)

Immunohistochemical analysis of Alpha-fetoglobulin staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

AFP

UniProt

P02771

NCBI Gene ID

174