AFP (A2) Mouse Monoclonal Antibody

• Catalog Number: orb4247
• Category: Antibodies
• Description: AFP (A2) Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: A2
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: AFP protein full length
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: AFP
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M PBS (pH7.4) with 0.02% Proclin300.
• Alternative names: alpha Fetoprotein; alpha-fetoprotein; alpha 1 feto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag at 2 µg/mL (100 µl/well) can bind Mouse Anti-Human AFP Antibody, the EC50 is 24.94 ng/ml.

25 ug total protein per lane of various lysates probed with AFP (A2) monoclonal antibody, unconjugated (orb4247) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AFP (A2)) Polyclonal Antibody, Unconjugated (orb4247) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868589) for 90 minutes, and DAPI for nuclei staining.