AFP (A4) Mouse Monoclonal Antibody

SKU: orb4246

Description

AFP (A4) Mouse Monoclonal Antibody

Research Area

Cancer Research, Cardiovascular Research, Cardiovascuolar, Liver Development

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA=1:5000-10000
• Reactivity: Human
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: A4
• Immunogen: AFP protein full length
• Target: AFP
• Molecular Weight: 65 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS (pH7.4) with 0.02% Proclin300.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

alpha Fetoprotein; alpha-fetoprotein; alpha 1 fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.

Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag at 2 µg/mL (100 µl/well) can bind Mouse Anti-Human AFP Antibody, the EC50 is 20.04 ng/ml.

Sample: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-AFP (A4) (orb4246) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.

Quick Database Links

Gene Symbol

AFP