AFP (A4) Mouse Monoclonal Antibody

• Catalog Number: orb4246
• Category: Antibodies
• Description: AFP (A4) Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: A4
• Tested applications: ELISA
• Predicted Reactivity: Human
• Reactivity: Human
• Isotype: IgG
• Immunogen: AFP protein full length
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: AFP
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M PBS (pH7.4) with 0.02% Proclin300.
• Alternative names: alpha Fetoprotein; alpha-fetoprotein; alpha 1 feto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag at 2 µg/mL (100 µl/well) can bind Mouse Anti-Human AFP Antibody, the EC50 is 20.04 ng/ml.

Sample: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-AFP (A4) (orb4246) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.