AFP/Alpha-fetoprotein Mouse Monoclonal Antibody

SKU: orb2960496

Description

Anti-Human AFP/Alpha-fetoprotein Antibody (BCH7) is a mouse monoclonal antibody detecting Alpha-fetoprotein in FCM, ICC, IF, WB. Suitable for Human. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, ICC, IF, WB
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: BCH7
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP

Flow-cytometry using anti-human AFP antibody. AFP Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human AFP antibody monoclonal antibody (Catalog # orb2960496, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using PE anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human AFP monoclonal antibody (Catalog: orb2960496, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human AFP/Alpha-fetoprotein Antibody.

Western blot analysis was performed using anti-AFP/Alpha-fetoprotein monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human AFP/Alpha-fetoprotein (Catalog No: orb2964280), Lane 2: negative control.

Quick Database Links

UniProt

P02771