AIF1 Antibody

• Catalog Number: orb1249312
• Category: Antibodies
• Description: AIF1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Porcine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-TGPPAKKAISELP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Brain (Frontal Cortex) and Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_001614.3 and 16.8kDa according to Rat NP_058892.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.This antibody can also be purchased as directly conjugated to HRP. Please specify when you order.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: AIF1
• UniProt ID: P55008
• NCBI: NP_001614.3, NP_116573.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: AIF1, IBLY409840A1, allograft inflammatory factor
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Brain (Frontal Cortex) and Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_001614.3 and 16.8kDa according to Rat NP_058892.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.This antibody can also be purchased as directly conjugated to HRP. Please specify when you order.
• Expiration Date: 12 months from date of receipt.

orb1249312 (1 ug/ml) staining of Human (A) and (0.5 ug/ml) of Mouse (B) and Rat (C) Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249312 (2 ug/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. This data is from a previous batch, not on sale.

orb1249312 (5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining. This data is from a previous batch, not on sale.

orb1249312 (5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1249312 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1249312 (5 ug/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.

orb1249312 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. This data is from a previous batch, not on sale.