AIF1 Antibody

SKU: orb1249312

Description

AIF1 Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Brain (Frontal Cortex) and Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_001614.3 and 16.8kDa according to Rat NP_058892.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.This antibody can also be purchased as directly conjugated to HRP. Please specify when you order.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-TGPPAKKAISELP
• Target: AIF1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

AIF1, IBLY409840A1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, DADB-70P7.8

orb1249312 (1 ug/ml) staining of Human (A) and (0.5 ug/ml) of Mouse (B) and Rat (C) Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249312 (2 ug/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. This data is from a previous batch, not on sale.

orb1249312 (5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining. This data is from a previous batch, not on sale.

orb1249312 (5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1249312 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1249312 (5 ug/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.

orb1249312 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. This data is from a previous batch, not on sale.

Quick Database Links

Gene Symbol

AIF1

UniProt

P55008

RefSeq (Protein):NP_116573.1, NP_001614.3